MinJoo Kim

Former master student

Bovine serum albumin-modified 3D printed alginate dialdehyde-gelatin scaffolds incorporating inorganic nanoparticles for bone regeneration

 

Supervisors: Dr. Farnaz Ghorbani, Prof. Aldo R. Boccaccini

Developing multi-material scaffolds that combine 3D printing technology and surface modification is crucial to achieving an efficient bone regeneration process. The objective of this master thesis is to develop bi-layer scaffolds by incorporating polydopamine [1] and silicate nanoparticle [2] complexes into 3D printed alginate dialdehyde-gelatin scaffolds [3], as well as functionalising the surface with a thin layer of bovine serum albumin [4]. Polydopamine and inorganic nanoparticles are expected to synergistically affect the scaffold’s bioactivity and osteogenic performance and improve their mechanical stability. Additionally, surface coating with protein is expected to regulate the system’s thermodynamic behaviour and facilitate tissue-scaffold interaction.

[1] Ghorbani, F.; Zamanian, A.; Behnamghader, A.; Joupari, M. D. A Facile Method to Synthesize Mussle-Inspired Polydopamine Nanopsheres as an Active Template for in Situ Formation of Biomimetic Hydroxyapatite. Materials Science and Engineering: C 2019, 94, 729-739.

[2] Monavari, M.; Homaeigohar, S.; Fuentes-Chandía, M.; Nawaz, Q.; Monavari, M.; Venkatraman, A.; Boccaccini, A. R. 3D Printing of Alginate-Dialdehyde-Gelatin (ADA-GEL) Hydrogels Incorporating Phytotherapeutic Icariin Loaded Mesoporous SiO2-CaO Nanoparticles for Bone Tissue Engineering. Materials Science and Engineering: C 2021, 131, 112470.

[3] Ghorbani, F.; Kim M.; Monavari M.; Ghalandari B.; Boccaccini A. R. Mussel-inspired polydopamine decorated alginate dialdehyde-gelatin 3D printed scaffolds for bone tissue engineering application. Frontiers in Bioengineering and Biotechnology 2022, doi: 10.3389/fbioe.2022.940070.

[4] Ghalandari, B.; Yu Y.; Ghorbani, F.; Warden, A. R.; Ahmad, K. Z.; Sang X.; Huang S.; Zhang Y.; Su W.; Divsalar A.; Ding X. Polydopamine nanospheres coated with bovine serum albumin permit enhance cell differentiation: fundamental mechanism and practical application for protein coating formation. Nanoscale 2021, 13, 20098.