Tanja Rosenberger

Bachelor student (Life Science Engineering)

Library development of ADA-GEL with various oxidation degrees for biofabrication and tissue engineering application

Supervisors: Hsuan-Heng Lu, Prof Aldo R. Boccaccini

Alginate dialdehyde (ADA) is a derivate of alginate [1]. Combined with gelatine it forms a hydrogel (ADA-GEL) which has good biocompatibility and high water binding capacity [2]. Therefore it is commonly used in biofabrication and tissue engineering. To enhance the stability and adjust the degradation of ADA-GEL, crosslinkers are used. Different oxidation degrees of ADA result in various viscosities, degradation behaviours, and cell material interactions [1]. In this Bachelor thesis the aim is to provide an overview of ADA-GELs with different degrees of oxidation in order to better determine which ADA-GEL hydrogel is suitable for determined purposes.

[1] S. Reakasame and A. R. Boccaccini , “Oxidized Alginate Based Hydrogels for Tissue Engineering Applications: A Review,” Biomacromolecules, vol. 19, no. 1. American Chemical Society, pp. 3 21, Jan. 08, 2018. doi: 10.1021/acs.biomac.7b01331.

[2] F. Bider, E. Karakaya, D. Mohn, and A. R. Boccaccini, “Advantages of nanoscale bioactive glass as inorganic filler in alginate hydrogels for drug delivery and biofabrication,” European Journal of Materials, vol. 2, no. 1, pp. 33 53, Dec. 2022, doi: 10.1080 26889277.2022.2039078.

[3] S. Heid et al., “Bioprinting with bioactive alginate dialdehyde gelatin (ADA GEL) composite bioinks: Time dependent in situ crosslinking via addition of calcium silicate particles tunes in vitro stability of 3D bioprinted constructs,” Bioprinting, vol. 26, Jun. 2022, doi: 10.1016/j.bprint.2022.e00200.