Tommi Bui

Master student

Evaluation of Drop-on-Demand Bioprinting using Alginate Di-Aldehyde Methacryloyl (ADAMA) and Gelatin Methacryloyl (GelMA)

Supervisors: Dr.-Ing. Rainer Detsch, Prof. Aldo R. Boccaccini

Drop-on-Demand Bioprinting is a precise technique for depositing bioink droplets, laden with cells, at high resolution and user-defined locations [1]. While previous studies have demonstrated commendable cell viability using this method, the existing bioinks need further improvements [2,3]. This work explores the potential of a multi-crosslinkable bioink, comprising ADAMA and GelMA for bioprinting of biological models and tissues. The formulation and processing of this highly adaptable functionalized bioink composition will be optimized and its properties will be analyzed. For the evaluation, spectroscopic methods, mechanical and printability tests as well as in vitro studies will be used.

[1] R. Detsch, et al., Evaluation of cell inkjet printing technique for biofabrication, BioNanoMaterials 17 (2016) 185–191

[2] E. Karakaya, et al., Targeted Printing of Cells: Evaluation of ADA-PEG Bioinks for Drop on Demand Approaches, Gels 8 (2022) 206

[3] S. Derakhshanfar, et. al., 3D bioprinting for biomedical devices and tissue engineering: A review of recent trends and advances, Bioact. Mater. 3 (2018) 144–156